Nelson:To Do - Sid

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==The List==
==The List==
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*make competent Xl1-blue cells
 
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*transform dsM13 into Xl1-blue
 
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*pour two SDS-PAGE gels (12%)
 
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*run fractions from the polg-b mutant on SDS-PAGE
 
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*spin down the cells in the shaker
 
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*make 6 L of LB and autoclave, plus 4 - 100 ml flasks
 
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*start overnight culture of RNAp (AMP/Chloro)
 

Revision as of 10:36, 10 April 2009

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