Neomycin: Difference between revisions
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==Experimental== | ==Experimental== | ||
===Target organism=== | |||
bacteria, mammalian cells | |||
===Stock solution=== | ===Stock solution=== | ||
10mg/ml in water | |||
===Working solution=== | ===Working solution=== | ||
10-50µg/ml | |||
==Description== | |||
===Mode of action=== | |||
Binds to ribosomal components and inhibits protein synthesis. | |||
===Mode of resistance=== | |||
''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | |||
==Related antibiotics== | |||
[[kanamycin]], [[G418]] | |||
== | ==External links== | ||
* list of cell culture reagents by Krackeler [http://www.krackeler.com/products/fid/2910] | |||
* list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14] | |||
* forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin] | |||
Revision as of 06:02, 4 August 2006
Experimental
Target organism
bacteria, mammalian cells
Stock solution
10mg/ml in water
Working solution
10-50µg/ml
Description
Mode of action
Binds to ribosomal components and inhibits protein synthesis.
Mode of resistance
neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.
