Neomycin: Difference between revisions
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==Experimental== | ==Experimental== | ||
'''target organism''': bacteria, mammalian cells | *'''target organism''': bacteria, mammalian cells | ||
'''stock solution''': 10mg/ml in water | *'''stock solution''': 10mg/ml in water | ||
'''working solution''': 10-50µg/ml (stable at RT for 5days) | *'''working solution''': 10-50µg/ml (stable at RT for 5days) | ||
==Description== | ==Description== | ||
'''mode of action''': binds to ribosomal components and inhibits protein synthesis. | *'''mode of action''': binds to ribosomal components and inhibits protein synthesis. | ||
'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | *'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | ||
==Links== | ==Links== | ||
Revision as of 06:07, 4 August 2006
Experimental
- target organism: bacteria, mammalian cells
- stock solution: 10mg/ml in water
- working solution: 10-50µg/ml (stable at RT for 5days)
Description
- mode of action: binds to ribosomal components and inhibits protein synthesis.
- mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.
