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  • target organism: bacteria, mammalian cells
  • stock solution: 10mg/ml in water
  • working solution: 10-50µg/ml (stable at RT for 5days)


  • mode of action: binds to ribosomal components and inhibits protein synthesis.
  • mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.


  • related antibiotics: kanamycin, G418
  • list of cell culture reagents by Krackeler [1]
  • list of cell culture reagents by USB [2]
  • forum thread on difference between neomycin & G418 [3]
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