Neutral red: Difference between revisions
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==Notes== | ==Notes== | ||
# | #242.5ml PBS + 7.5ml Stock Neutral Red = 250ml Working Stock Neutral Red | ||
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or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | ||
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[[Category:In vitro]] | [[Category:In vitro]] | ||
Latest revision as of 04:13, 19 February 2009
Overview
Neutral Red for gHV68 (DW)
Materials
- Neutral Red
- Whatman filter paper
- Bottles
Procedure
Stock Solution:
- Add 25g neutral red to 2 liters water.
- Incubate o/n at 56C protected from light.
- Filter through Whatman filter paper.
- Aliquot into small bottles and wrap in tin foil.
- Autoclave.
- Store stock at RT.
Working solution:
- Dilute 3 ml stock solution per 100 ml PBS.
- Filter.
- Apply to methylcellulose.
Notes
- 242.5ml PBS + 7.5ml Stock Neutral Red = 250ml Working Stock Neutral Red
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
