Nick Rohacz: Week 2

Make a list of at least 10 biological terms for which you did not know the definitions when you first read the article. Define each of the terms. You can use the glossary in any molecular biology, cell biology, or genetics text book as a source for definitions, or you can use one of many available online biological dictionaries (links below). List the citation(s) for the dictionary(s) you use, providing a URL to the page is fine.

1. Dilution rate - A solution that is diluted per unit of time, in this case .15/hr.[1]
2. Ammonia Limitations - That which limits; a restriction; qualification; a restraining condition. [2]
3. Ammonia Flux - Total amount of ammonia passing through a given surface over a unit of time. [3]
4. Oligonucleotide - An oligonucleotide" is a short nucleotide sequence, generally made up of less than 20 nucleotides. [4]
5. Amino acid starvation - Lengthy and continuous deprivation of Amino Acids. [5]
6. Two-component sensing system - A regulatory system that controls gene expression in response to nitrogen-source availability. [6]
7. Gram-negative bacteria - Bacteria which lose crystal violet stain but are stained pink when treated by grams method. [7]
8. Proline - One of the 20 amino acids directly coded for in proteins. [8]
9. Biomass - The total mass of all living material in a specific area, habitat, or region. [9]
10. Northern RNA analysis- An electroblotting method in which rNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or dNA. [10]

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Write an outline of the article. The length should be the equivalent of 2 pages of standard 8 1/2 by 11 inch paper. Your outline can be in any form you choose, but you should utilize the wiki syntax of headers and either numbered or bulleted lists to create it. The text of the outline does not have to be complete sentences, but it should answer the questions listed below and have enough information so that others can follow it. However, your outline should be in YOUR OWN WORDS, not copied straight from the article.

   * What is the main result presented in this paper?
   * What is the importance or significance of this work?
   * What were the limitations in previous studies that led them to perform this work?
   * What were the methods used in the study?
   * Briefly state the result shown in each of the figures.
         o What do the X and Y axes represent?
         o How were the measurements made?
         o What trends are shown by the plots and what conclusions can you draw from the data? 
   * What is the overall conclusion of the study and what are some future directions for research?