Nick Rohacz: Week 4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 1: | Line 1: | ||
==Terminology== | ==Terminology== | ||
# | #The 'Michealis-Menten model of enzyme kinetics' is a model used to measure the concentrations of enzymes, product, and substrate using rate laws and the model enzyme + substrate ↔ enzyme/substrate → enzyme + product. | ||
# | #The 'experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten' produces a linear form of Michealis Menten where K is the slope/intercept and Vmax is 1/intercept. | ||
# | #A 'Chemostat Reactor' is a reactor in which the conditions are easily controlled by keeping homeostasis througha static equilibrium, thus allowing controlled growth of the organism. | ||
# | #'Exponential Growth' is when the output of a model is not linear with the input, but rather the output far exceeds the amount input. Similar to the graph y=e<sup>x</sup>. | ||
==Applying Michealis-Menten Models== | ==Applying Michealis-Menten Models== | ||
==Estimating Michealis-Menten Parameters== | ==Estimating Michealis-Menten Parameters== | ||
Slope = 859.23 | Slope = 859.23 | ||
| Line 12: | Line 13: | ||
[[User:Nicholas A. Rohacz|Nicholas A. Rohacz]] 23:24, 6 February 2011 (EST) | [[User:Nicholas A. Rohacz|Nicholas A. Rohacz]] 23:24, 6 February 2011 (EST) | ||
{{Template:Nicholas_A._Rohacz}} | |||
Revision as of 23:22, 6 February 2011
Terminology
- The 'Michealis-Menten model of enzyme kinetics' is a model used to measure the concentrations of enzymes, product, and substrate using rate laws and the model enzyme + substrate ↔ enzyme/substrate → enzyme + product.
- The 'experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten' produces a linear form of Michealis Menten where K is the slope/intercept and Vmax is 1/intercept.
- A 'Chemostat Reactor' is a reactor in which the conditions are easily controlled by keeping homeostasis througha static equilibrium, thus allowing controlled growth of the organism.
- 'Exponential Growth' is when the output of a model is not linear with the input, but rather the output far exceeds the amount input. Similar to the graph y=ex.
Applying Michealis-Menten Models
Estimating Michealis-Menten Parameters
Slope = 859.23 Intercept = 31.695 Vmax = 1/intercept = .03155 K = slope/intercept = 27.109
Nicholas A. Rohacz 23:24, 6 February 2011 (EST)
Class Assignments
Summer Research
Class Journals
