Nick Rohacz: Week 4: Difference between revisions
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==Terminology== | ==Terminology== | ||
# | #The ''Michealis-Menten model of enzyme kinetics'' is a model used to measure the concentrations of enzymes, product, and substrate using rate laws and the model enzyme + substrate ↔ enzyme/substrate → enzyme + product. | ||
# | #The ''experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten'' produces a linear form of Michealis Menten where K is the slope/intercept and Vmax is 1/intercept. | ||
# | #A ''Chemostat Reactor'' is a reactor in which the conditions are easily controlled by keeping homeostasis througha static equilibrium, thus allowing controlled growth of the organism. | ||
# | #''Exponential Growth'' is when the output of a model is not linear with the input, but rather the output far exceeds the amount input. Similar to the graph y=e<sup>x</sup>. | ||
==Applying Michealis-Menten Models== | ==Applying Michealis-Menten Models== | ||
[[Image:NAR_CAT.jpg]] | |||
[[Image:NAR_CAT2.jpg]] | |||
[[Image:NAR_CAT3.jpg]] | |||
==Estimating Michealis-Menten Parameters== | ==Estimating Michealis-Menten Parameters== | ||
Slope = 859.23 | Slope = 859.23 | ||
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[[User:Nicholas A. Rohacz|Nicholas A. Rohacz]] 23:24, 6 February 2011 (EST) | [[User:Nicholas A. Rohacz|Nicholas A. Rohacz]] 23:24, 6 February 2011 (EST) | ||
{{Template:Nicholas_A._Rohacz}} | |||
Latest revision as of 00:14, 7 February 2011
Terminology
- The Michealis-Menten model of enzyme kinetics is a model used to measure the concentrations of enzymes, product, and substrate using rate laws and the model enzyme + substrate ↔ enzyme/substrate → enzyme + product.
- The experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten produces a linear form of Michealis Menten where K is the slope/intercept and Vmax is 1/intercept.
- A Chemostat Reactor is a reactor in which the conditions are easily controlled by keeping homeostasis througha static equilibrium, thus allowing controlled growth of the organism.
- Exponential Growth is when the output of a model is not linear with the input, but rather the output far exceeds the amount input. Similar to the graph y=ex.
Applying Michealis-Menten Models
Estimating Michealis-Menten Parameters
Slope = 859.23 Intercept = 31.695 Vmax = 1/intercept = .03155 K = slope/intercept = 27.109
Nicholas A. Rohacz 23:24, 6 February 2011 (EST)
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