Nick Rohacz: Week 8: Difference between revisions
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*5)[[Image:Gene_comparison_NAR.jpg]] | *5)[[Image:Gene_comparison_NAR.jpg]] | ||
**Used numbers instead of colors for graphing. | |||
***Bright Red = 20 | ***Bright Red = 20 | ||
***Bright-Medium Red = 15 | ***Bright-Medium Red = 15 | ||
| Line 11: | Line 11: | ||
***Bright Green = -20(not present) | ***Bright Green = -20(not present) | ||
*6b)From 1 hour to 9 hours, respectively. | *6b)From 1 hour to 9 hours, respectively. | ||
Gene X: black, medium red, black, medium red | **Gene X: black, medium red, black, medium red | ||
Gene Y: black, bright red, dim green, green | **Gene Y: black, bright red, dim green, green | ||
Gene Z: black, dim red, medium red, medium red | **Gene Z: black, dim red, medium red, medium red | ||
*7) Genes X and Y both started with no change in activity at 1 hour, both increased their activity in the oxygen limited group at 3 hour, reduced back to no change in activity at 5 hours, and decreased their activity in the oxygen limited group at 7 hours. | *7) Genes X and Y both started with no change in activity at 1 hour, both increased their activity in the oxygen limited group at 3 hour, reduced back to no change in activity at 5 hours, and decreased their activity in the oxygen limited group at 7 hours. | ||
*9) Because the activity is the same in both the experimental and control groups at the beginning, that is, when they both have enough oxygen. | *9) Because the activity is the same in both the experimental and control groups at the beginning, that is, when they both have enough oxygen. | ||
Revision as of 23:27, 13 March 2011
- 5)
- Used numbers instead of colors for graphing.
- Bright Red = 20
- Bright-Medium Red = 15
- Medium Red = 10
- Dim Red = 3(not present)
- Black = 0
- Dim Green = -3(not present)
- Medium Green = -10(not present)
- Bright-Medium Green = 15(not present)
- Bright Green = -20(not present)
- Used numbers instead of colors for graphing.
- 6b)From 1 hour to 9 hours, respectively.
- Gene X: black, medium red, black, medium red
- Gene Y: black, bright red, dim green, green
- Gene Z: black, dim red, medium red, medium red
- 7) Genes X and Y both started with no change in activity at 1 hour, both increased their activity in the oxygen limited group at 3 hour, reduced back to no change in activity at 5 hours, and decreased their activity in the oxygen limited group at 7 hours.
- 9) Because the activity is the same in both the experimental and control groups at the beginning, that is, when they both have enough oxygen.
- 10) As discussed in the paper, "...as if [the cells] sensed the glucose concentration was being reduced and thus might soon starve. As a result genes in entire pathways were induced or repressed. Genes involved in protein synthesis were coordinately repressed" (Campbell and Heyer 120). And since TEF4 is a translational elongation factor, meaning it adds amino acids to the polypeptide chain, this is a protein synthesizing gene and is therefore reduced.
- 11) Because the TCA cycle shares a common promoter with another gene that is induced by a lack of glucose, therefore the TCA cycle would also be induced, causing the creation of energy in the yeast cell, through the electron transport chain, so that it can survive with no more glucose present.
- 12) One mechanism is that any other genes that share a common promoter or other transcription factor, would expressed in a similar matter to the gene in question.
- 13) Red, because the cell would eventually use up all of its glucose in every process, then it would starve to death.
- 14) Red, because an overexpression of Yap1 would cause an increased resistance to all environmental stresses present in the cell.
- 15) If the repressor was needed to repress one gene so another can be induced, or vice versa, then yes, the loss of a repressor or overexpression of a transcription factor could cause the repression of a gene.
- 16) Green spots should be present, this can be verified by comparing the gene to another gene that shares its promoter site, these genes should also be repressed/green.
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