View source for Nikon TE2000 Microscope

==Equipment==
===CCDD Camera===
[http://microscopyu.com/articles/digitalimaging/digitalintro.html Digital Imaging with CCD Camera]

This [http://microscopyu.com/tutorials/flash/pixelcalc/index.html page] explains why the 60X is as good as the 100X objective for our camera.

[[Methods to determine the size of an object in microns]]

===Condenser===
[[Focusing the Nikon TE2000 Condenser|Focusing the condenser]]

===FL Lamp===
*It takes the lamp about 2 minutes to warm up.
*After a lamp has been turned off, it is a good idea to wait 30 min. before turning on again.

===Objectives===
We have the following objectives:

====Dry Objectives====
*2X
*10X
*20X
*40X

====Oil Objectives====
*60X
*100X

(need to add the other features of these objectives, NA, working distance, etc.)

===Filter Sets===

[[FITC/GFP Filter Set]]

[[CFP Filter Set]]

[[YFP Filter Set]]

[[RFP Filter Set]]

[[UV/DAPI Filter Set]]

===Solent incubation chamber===

[[Endy:Changing the sensor zero value on Solent incubator|Changing the sensor zero value on Solent incubator]]

===Power Mac G5 Dual Processor===
'''Internal IP address''' - 192.168.4.102

==Protocols==

[[Quantitative Microscopy]]

[[How to reduce agarose pad sliding]]

[[Getting started on the microscope|Help, I can't find my cells!]]

==IPLab Scripts==
[[Autofocus IPLab Scripts]]

[[High-throughput Imaging IPLab Scripts]]

[[Movie-making IPLab Scripts]]

==Image editing==
===For Mac OS X===
Only "Graphics converter" and "Image J" can read the IPLab images directly. For ImageJ to open those images, you need to install a macro. Go to the Plugins Menu, choose "Macro", then "Install". Install the macro named OpenMacIPLab. The description of the file IPLab Mac format is in the User's Guide (Appendix), Other programs with a configurable "import" command may be able to open them once an specific configuration is set.

==Links==
*[http://microscopyu.com/ Nikon MicroscopyU] is a useful resource for learning more about the optics relevant to the scope.
*[http://microscopy.fsu.edu/ Molecular Expressions] has general information related to microscopy.  Some of this information appears to the the same as that offered by MicroscopyU.  <br> ''Note: one of the more useful parts of these two site are the Javascript tutorials.  Unfortunately, they don't seem to load under Mac OS 10.3 using either Firefox or Safari.  They do work in Tiger 10.4 using Safari however.  If anyone knows of a workaround in 10.3, please place a link or note here.''
*[http://www.mathworks.com/access/helpdesk/help/toolbox/images/images_product_page.html Image processing toolbox] from [http://www.mathworks.com/ Matlab].

==Issues==
[[Endy:Nikon TE2000 microscope service history|Service history]]

==Troubleshooting==
Q:Someone mistakenly put oil on the 40X dry objective.  What's the best
procedure for cleaning that off, without causing damage to the lens?

A(from Barry Alpert):Use either ether or chloroform with a wooden handled q tip ( I know thw lab supply houses carry this item )and gently wipe the oil off the lens. DO NOT scrub , as it will damage the coating.

==Contact==
[[Jason Kelly]]