Nikon TE2000 Microscope

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==Protocols==
==Protocols==
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[[Pixel calibration]] (to determine the pixel-um correspondance for a given objective)
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[[Quantitative Microscopy]]
[[Quantitative Microscopy]]

Revision as of 21:45, 16 June 2005

Contents

Equipment

CCDD Camera

Digital Imaging with CCD Camera

This page explains why the 60X is as good as the 100X objective for our camera.

Condenser

Focusing the condenser

Objectives

We have the following objectives:

Dry Objectives

  • 2X
  • 10X
  • 20X
  • 40X

Oil Objectives

  • 60X
  • 100X

(need to add the other features of these objectives, NA, working distance, etc.)

Filter Sets

FITC/GFP Filter Set

CFP Filter Set

YFP Filter Set

RFP Filter Set

UV/DAPI Filter Set

Protocols

Pixel calibration (to determine the pixel-um correspondance for a given objective)

Quantitative Microscopy

How to reduce agarose pad sliding

IPLab Scripts

Autofocus IPLab Scripts

High-throughput Imaging IPLab Scripts

Movie-making IPLab Scripts

Links

Nikon MicroscopyU is a useful resource for learning more about the optics relevant to the scope.

Personal tools