Nikon TE2000 Microscope

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[[Movie-making IPLab Scripts]]
[[Movie-making IPLab Scripts]]
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==Image editing==
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===For Mac OS X===
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Only "Graphics converter" and "Image J" can read the IPLab images directly. For ImageJ to open those images, you need to install a macro. Go to the Plugins Menu, choose "Macro", then "Install". Install the macro named OpenMacIPLab. The description of the file IPLab Mac format is in the User's Guide (Appendix), Other programs with a configurable "import" command may be able to open them once an specific configuration is set.
==Links==
==Links==
[http://microscopyu.com/ Nikon MicroscopyU] is a useful resource for learning more about the optics relevant to the scope.
[http://microscopyu.com/ Nikon MicroscopyU] is a useful resource for learning more about the optics relevant to the scope.

Revision as of 13:15, 17 June 2005

Contents

Equipment

CCDD Camera

Digital Imaging with CCD Camera

This page explains why the 60X is as good as the 100X objective for our camera.

Condenser

Focusing the condenser

Objectives

We have the following objectives:

Dry Objectives

  • 2X
  • 10X
  • 20X
  • 40X

Oil Objectives

  • 60X
  • 100X

(need to add the other features of these objectives, NA, working distance, etc.)

Filter Sets

FITC/GFP Filter Set

CFP Filter Set

YFP Filter Set

RFP Filter Set

UV/DAPI Filter Set

Protocols

Pixel size calibration (to determine the pixel-um correspondance for a given objective)

Quantitative Microscopy

How to reduce agarose pad sliding

IPLab Scripts

Autofocus IPLab Scripts

High-throughput Imaging IPLab Scripts

Movie-making IPLab Scripts

Image editing

For Mac OS X

Only "Graphics converter" and "Image J" can read the IPLab images directly. For ImageJ to open those images, you need to install a macro. Go to the Plugins Menu, choose "Macro", then "Install". Install the macro named OpenMacIPLab. The description of the file IPLab Mac format is in the User's Guide (Appendix), Other programs with a configurable "import" command may be able to open them once an specific configuration is set.

Links

Nikon MicroscopyU is a useful resource for learning more about the optics relevant to the scope.

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