Nikon TE2000 Microscope

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==Protocols==
==Protocols==
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[[Pixel size calibration]] (to determine the pixel-um correspondance for a given objective)
 
[[Quantitative Microscopy]]
[[Quantitative Microscopy]]

Revision as of 21:22, 14 August 2005

Contents

Equipment

CCDD Camera

Digital Imaging with CCD Camera

This page explains why the 60X is as good as the 100X objective for our camera.

Methods to determine the size of an object in microns

Condenser

Focusing the condenser

Objectives

We have the following objectives:

Dry Objectives

  • 2X
  • 10X
  • 20X
  • 40X

Oil Objectives

  • 60X
  • 100X

(need to add the other features of these objectives, NA, working distance, etc.)

Filter Sets

FITC/GFP Filter Set

CFP Filter Set

YFP Filter Set

RFP Filter Set

UV/DAPI Filter Set

Protocols

Quantitative Microscopy

How to reduce agarose pad sliding

IPLab Scripts

Autofocus IPLab Scripts

High-throughput Imaging IPLab Scripts

Movie-making IPLab Scripts

Image editing

For Mac OS X

Only "Graphics converter" and "Image J" can read the IPLab images directly. For ImageJ to open those images, you need to install a macro. Go to the Plugins Menu, choose "Macro", then "Install". Install the macro named OpenMacIPLab. The description of the file IPLab Mac format is in the User's Guide (Appendix), Other programs with a configurable "import" command may be able to open them once an specific configuration is set.

Links

  • Nikon MicroscopyU is a useful resource for learning more about the optics relevant to the scope.
  • Molecular Expressions has general information related to microscopy. Some of this information appears to the the same as that offered by MicroscopyU.
    Note: one of the more useful parts of these two site are the Javascript tutorials. Unfortunately, they don't seem to load under Mac OS 10.3 using either Firefox or Safari. They do work in Tiger 10.4 using Safari however. If anyone knows of a workaround in 10.3, please place a link or note here.
  • Image processing toolbox from Matlab.
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