Nikon TE2000 Microscope - Revision history

Stpierre: /* Objectives */

2009-04-02T20:35:43Z

Objectives

←Older revision		Revision as of 20:35, 2 April 2009
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	===Objectives===		===Objectives===
	We have the following objectives:		We have the following objectives:
		+	(It is unclear whether the item numbers specify the part number for Nikon, or only for MVI, a Nikon rep in the Boston area)

	====Dry Objectives====		====Dry Objectives====
-	*2x: Plan APO 2x/0.1	+	*2x: Plan APO 2x/0.1, Item
	*10x DIC: Plan Fluor 10x/0.3 DIC L/N1		*10x DIC: Plan Fluor 10x/0.3 DIC L/N1
-	*20x DIC: Plan Fluor 20x/0.5 DIC M/N2	+	*20x DIC: Plan Fluor 20x/0.5 DIC M/N2, Item #MRH00200
	*40X DIC: (add info here)		*40X DIC: (add info here)

	====Oil Objectives====		====Oil Objectives====
-	* 60x DIC: Plan APO 60x/1.4 DIC Oil	+	* 60x DIC: Cfi Plan APO 60x/1.4 DIC Oil, Item #MRD01602
-	* 60x phase contrast: Plan APO VC 60x/1.4 Oil Ph3 DM	+	* 60x phase contrast: Plan APO VC 60x/1.4 Oil Ph3 DM, Item #MRD31602
-	* 100x DIC: Plan APO 100x/1.4 Oil DIC H	+	* 100x DIC: Cfi Plan APO 100x/1.4 Oil DIC H, Item #MRD01900

	(need to add the other features of these objectives, NA, working distance, etc.)		(need to add the other features of these objectives, NA, working distance, etc.)

2009-04-02T20:30:33Z

Objectives

←Older revision		Revision as of 20:30, 2 April 2009
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	====Dry Objectives====		====Dry Objectives====
-	*2X	+	*2x: Plan APO 2x/0.1
-	*~~10X~~	+	*10x DIC: Plan Fluor 10x/0.3 DIC L/N1
-	*~~20X~~	+	*20x DIC: Plan Fluor 20x/0.5 DIC M/N2
-	*40X	+	*40X DIC: (add info here)

	====Oil Objectives====		====Oil Objectives====
-	*~~60X~~ DIC -- 1.~~40NA~~	+	* 60x DIC: Plan APO 60x/1.4 DIC Oil
-	*~~60X Phase Contrast --~~ 1.~~40NA~~	+	* 60x phase contrast: Plan APO VC 60x/1.4 Oil Ph3 DM
-	*~~100X~~ DIC -- 1.~~40NA~~	+	* 100x DIC: Plan APO 100x/1.4 Oil DIC H

	(need to add the other features of these objectives, NA, working distance, etc.)		(need to add the other features of these objectives, NA, working distance, etc.)

2007-05-18T04:01:02Z

Installation

←Older revision		Revision as of 04:01, 18 May 2007
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	###TE2 Filter Cube Turret		###TE2 Filter Cube Turret
	###TE2 Analyzer		###TE2 Analyzer
		+	###TE2 Optical Path (or something like that)
	#Check your scripts to make sure that they are calling the correct Positioners (e.g. you are opening Ludl Shutter 1 when you want a FL image, etc.)		#Check your scripts to make sure that they are calling the correct Positioners (e.g. you are opening Ludl Shutter 1 when you want a FL image, etc.)
	#Make sure that the camera is set to 12 bit by going to Camera -> Camera Settings -> Bit Depth = 12.		#Make sure that the camera is set to 12 bit by going to Camera -> Camera Settings -> Bit Depth = 12.
		+	# To ensure that all users have the same setup, move the following files from administrator -> Library -> Preferences to the corresponding folders in the desired user folder:
		+	##DCAM.plist
		+	##com.biovis.ivisionmac.app.plist
		+	#Also, copy everything from iVision user folder in the administrator account into the user iVision user folder.

	==Protocols==		==Protocols==

2007-05-11T23:34:01Z

Installation

←Older revision		Revision as of 23:34, 11 May 2007
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	##Select the appropriate port, and hit Ok, then connect.		##Select the appropriate port, and hit Ok, then connect.
	#Open the Control -> Device Select menu		#Open the Control -> Device Select menu
-		+	##Under '''Stages''', select the following:
		+	###XY Stage: Ludl Stage
		+	###Z axis: Nikon TE2000 Z-axis
		+	##Under ''Microscope'', select the following "Positioners":
		+	###Ludl Shutter 1
		+	###Ludl Shutter 2
		+	###TE2 Filter Cube Turret
		+	###TE2 Analyzer
		+	#Check your scripts to make sure that they are calling the correct Positioners (e.g. you are opening Ludl Shutter 1 when you want a FL image, etc.)
		+	#Make sure that the camera is set to 12 bit by going to Camera -> Camera Settings -> Bit Depth = 12.

	==Protocols==		==Protocols==

2007-05-11T23:22:26Z

←Older revision		Revision as of 23:22, 11 May 2007
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	===Power Mac G5 Dual Processor===		===Power Mac G5 Dual Processor===
	'''Internal IP address''' - 192.168.4.102		'''Internal IP address''' - 192.168.4.102
		+
		+	==Installation==
		+
		+	#Install IPLab from IPLab CD
		+	#Install all other files on IPLab CD (Camera Control, Motion control, etc.)
		+	#In the folder Applications -> IPLab 3.9 Folder -> Device Modules, move all devce modules to the Device Modules - Disabled folder '''EXCEPT''':
		+	##Ludl S&F.ipmd
		+	##Ludl Stage.ipmd
		+	##Nikon TE2000.ipmd
		+	##Orca-DCAM.ipma
		+	##Virtual Camera.ipma
		+	#Install Keyspan software from Keyspan CD
		+	#Make sure that the switch box, camera, and microscope are all plugged into the Mac.
		+	#Open IPLab
		+	#If the window doesn't open up automatically, open the menu Control -> Device Setup
		+	##You should see the following:
		+	###Cameras: Orca-DCAM and VirtualCam
		+	###Stages: Ludl Stage and Nikon TE2000 Z-Axis
		+	###Positioners: Ludl S&F and Nikon E2000 Light Path
		+	##If any of the following are listed at triangles, select them and hit Setup
		+	##Select the appropriate port, and hit Ok, then connect.
		+	#Open the Control -> Device Select menu
		+

	==Protocols==		==Protocols==

2007-03-14T20:27:16Z

Contact

←Older revision		Revision as of 20:27, 14 March 2007
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	==Contact==		==Contact==
	[[Jason Kelly]]		[[Jason Kelly]]
		+
		+	[[Category:Equipment]] [[Category:Microscopy]]

2006-11-02T18:25:40Z

Equipment

←Older revision		Revision as of 18:25, 2 November 2006
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	[http://microscopyu.com/articles/digitalimaging/digitalintro.html Digital Imaging with CCD Camera]		[http://microscopyu.com/articles/digitalimaging/digitalintro.html Digital Imaging with CCD Camera]

-	This [http://microscopyu.com/tutorials/flash/pixelcalc/index.html page] explains why the 60X is as good as the 100X objective for our camera.	+	This [http://microscopyu.com/tutorials/flash/pixelcalc/index.html page] and this [http://www.microscopyu.com/tutorials/java/digitalimaging/pixelcalculator/index.html page] explains why the 60X is almost as good as the 100X objective for our camera.

	[[Methods to determine the size of an object in microns]]		[[Methods to determine the size of an object in microns]]

2006-11-02T18:16:49Z

Oil Objectives

←Older revision		Revision as of 18:16, 2 November 2006
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	====Oil Objectives====		====Oil Objectives====
-	*60X	+	*60X DIC -- 1.40NA
-	*100X	+	*60X Phase Contrast -- 1.40NA
		+	*100X DIC -- 1.40NA

	(need to add the other features of these objectives, NA, working distance, etc.)		(need to add the other features of these objectives, NA, working distance, etc.)

2006-11-02T18:16:16Z

CCD Camera

←Older revision		Revision as of 18:16, 2 November 2006
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	[[Image:Endy NikonTE2000.jpg\|500px]]		[[Image:Endy NikonTE2000.jpg\|500px]]
	===CCD Camera===		===CCD Camera===
		+	Hamamatsu Digital CCD Camera C4742-80-12AG -- [http://www.sales.hamamatsu.com/en/products/system-division/cameras/all-uv-vis-ir-cameras/c4742-80-12ag-.php ORCA-AG Deep Cooled Digital Camera]
		+
	[http://microscopyu.com/articles/digitalimaging/digitalintro.html Digital Imaging with CCD Camera]		[http://microscopyu.com/articles/digitalimaging/digitalintro.html Digital Imaging with CCD Camera]

2006-10-29T20:54:33Z

←Older revision		Revision as of 20:54, 29 October 2006
Line 1:		Line 1:
	==Equipment==		==Equipment==
		+
		+	[[Image:Endy NikonTE2000.jpg\|500px]]
	===CCD Camera===		===CCD Camera===
	[http://microscopyu.com/articles/digitalimaging/digitalintro.html Digital Imaging with CCD Camera]		[http://microscopyu.com/articles/digitalimaging/digitalintro.html Digital Imaging with CCD Camera]