Nikon TE2000 Microscope
Equipment
CCD Camera
Hamamatsu Digital CCD Camera C4742-80-12AG -- ORCA-AG Deep Cooled Digital Camera
Digital Imaging with CCD Camera
This page and this page explains why the 60X is almost as good as the 100X objective for our camera.
Methods to determine the size of an object in microns
Condenser
FL Lamp
- It takes the lamp about 2 minutes to warm up.
- After a lamp has been turned off, it is a good idea to wait 30 min. before turning on again.
Objectives
We have the following objectives:
Dry Objectives
- 2X
- 10X
- 20X
- 40X
Oil Objectives
- 60X DIC -- 1.40NA
- 60X Phase Contrast -- 1.40NA
- 100X DIC -- 1.40NA
(need to add the other features of these objectives, NA, working distance, etc.)
Filter Sets
Solent incubation chamber
Changing the sensor zero value on Solent incubator
Power Mac G5 Dual Processor
Internal IP address - 192.168.4.102
Installation
- Install IPLab from IPLab CD
- Install all other files on IPLab CD (Camera Control, Motion control, etc.)
- In the folder Applications -> IPLab 3.9 Folder -> Device Modules, move all devce modules to the Device Modules - Disabled folder EXCEPT:
- Ludl S&F.ipmd
- Ludl Stage.ipmd
- Nikon TE2000.ipmd
- Orca-DCAM.ipma
- Virtual Camera.ipma
- Install Keyspan software from Keyspan CD
- Make sure that the switch box, camera, and microscope are all plugged into the Mac.
- Open IPLab
- If the window doesn't open up automatically, open the menu Control -> Device Setup
- You should see the following:
- Cameras: Orca-DCAM and VirtualCam
- Stages: Ludl Stage and Nikon TE2000 Z-Axis
- Positioners: Ludl S&F and Nikon E2000 Light Path
- If any of the following are listed at triangles, select them and hit Setup
- Select the appropriate port, and hit Ok, then connect.
- You should see the following:
- Open the Control -> Device Select menu
- Under Stages, select the following:
- XY Stage: Ludl Stage
- Z axis: Nikon TE2000 Z-axis
- Under Microscope, select the following "Positioners":
- Ludl Shutter 1
- Ludl Shutter 2
- TE2 Filter Cube Turret
- TE2 Analyzer
- Under Stages, select the following:
- Check your scripts to make sure that they are calling the correct Positioners (e.g. you are opening Ludl Shutter 1 when you want a FL image, etc.)
- Make sure that the camera is set to 12 bit by going to Camera -> Camera Settings -> Bit Depth = 12.
Protocols
How to reduce agarose pad sliding
IPLab Scripts
High-throughput Imaging IPLab Scripts
Image editing
For Mac OS X
Only "Graphics converter" and "Image J" can read the IPLab images directly. For ImageJ to open those images, you need to install a macro. Go to the Plugins Menu, choose "Macro", then "Install". Install the macro named OpenMacIPLab. The description of the file IPLab Mac format is in the User's Guide (Appendix), Other programs with a configurable "import" command may be able to open them once an specific configuration is set.
Links
- Nikon MicroscopyU is a useful resource for learning more about the optics relevant to the scope.
- Molecular Expressions has general information related to microscopy. Some of this information appears to the the same as that offered by MicroscopyU.
Note: one of the more useful parts of these two site are the Javascript tutorials. Unfortunately, they don't seem to load under Mac OS 10.3 using either Firefox or Safari. They do work in Tiger 10.4 using Safari however. If anyone knows of a workaround in 10.3, please place a link or note here. - Image processing toolbox from Matlab.
Issues
Troubleshooting
Q:Someone mistakenly put oil on the 40X dry objective. What's the best procedure for cleaning that off, without causing damage to the lens?
A(from Barry Alpert):Use either ether or chloroform with a wooden handled q tip ( I know thw lab supply houses carry this item )and gently wipe the oil off the lens. DO NOT scrub , as it will damage the coating.
