Nikon TE2000 Microscope

From OpenWetWare

Revision as of 14:16, 2 November 2006 by Skosuri (Talk | contribs)
Jump to: navigation, search

Contents

Equipment

CCD Camera

Hamamatsu Digital CCD Camera C4742-80-12AG -- ORCA-AG Deep Cooled Digital Camera

Digital Imaging with CCD Camera

This page explains why the 60X is as good as the 100X objective for our camera.

Methods to determine the size of an object in microns

Condenser

Focusing the condenser

FL Lamp

  • It takes the lamp about 2 minutes to warm up.
  • After a lamp has been turned off, it is a good idea to wait 30 min. before turning on again.

Objectives

We have the following objectives:

Dry Objectives

  • 2X
  • 10X
  • 20X
  • 40X

Oil Objectives

  • 60X
  • 100X

(need to add the other features of these objectives, NA, working distance, etc.)

Filter Sets

FITC/GFP Filter Set

CFP Filter Set

YFP Filter Set

RFP Filter Set

UV/DAPI Filter Set

Solent incubation chamber

Changing the sensor zero value on Solent incubator

Power Mac G5 Dual Processor

Internal IP address - 192.168.4.102

Protocols

Quantitative Microscopy

How to reduce agarose pad sliding

Help, I can't find my cells!

IPLab Scripts

Autofocus IPLab Scripts

High-throughput Imaging IPLab Scripts

Movie-making IPLab Scripts

Image editing

For Mac OS X

Only "Graphics converter" and "Image J" can read the IPLab images directly. For ImageJ to open those images, you need to install a macro. Go to the Plugins Menu, choose "Macro", then "Install". Install the macro named OpenMacIPLab. The description of the file IPLab Mac format is in the User's Guide (Appendix), Other programs with a configurable "import" command may be able to open them once an specific configuration is set.

Links

  • Nikon MicroscopyU is a useful resource for learning more about the optics relevant to the scope.
  • Molecular Expressions has general information related to microscopy. Some of this information appears to the the same as that offered by MicroscopyU.
    Note: one of the more useful parts of these two site are the Javascript tutorials. Unfortunately, they don't seem to load under Mac OS 10.3 using either Firefox or Safari. They do work in Tiger 10.4 using Safari however. If anyone knows of a workaround in 10.3, please place a link or note here.
  • Image processing toolbox from Matlab.

Issues

Service history

Troubleshooting

Q:Someone mistakenly put oil on the 40X dry objective. What's the best procedure for cleaning that off, without causing damage to the lens?

A(from Barry Alpert):Use either ether or chloroform with a wooden handled q tip ( I know thw lab supply houses carry this item )and gently wipe the oil off the lens. DO NOT scrub , as it will damage the coating.

Contact

Jason Kelly

Personal tools