Nissl staining: Difference between revisions
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== Target of the dye == | == Target of the dye == | ||
Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus. | Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus and other accumulations of nucleic acid. | ||
== External links == | == External links == | ||
* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia] | * [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia] | ||
* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington] | * [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington] | ||
Revision as of 04:46, 20 May 2010
The Nissl staining is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.
Principle
The Nissl staining employs a mixture of basic dyes (aniline, thionine, or cresyl violet) which stains negatively charged RNA.
Target of the dye
Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus and other accumulations of nucleic acid.
