Notebook:Barry Canton/PCR boilerplate

Not sure if this is going to be useful but we'll see.

Reaction 1

Reaction Mix (μl)		Thermocycling
H2O	39 Buffer= 5 Thermo Pol PrimerF= 1.5 R0183rRNAF PrimerR= 1.5 rRNA-4RTHR1/rRNA-4RTHR2 dNTPS= 2 Template= 1 1/100 of template prep Polymerase= Vent Denaturing Temp= 95 Denaturing Time= 1min Denaturing Cycle Temp= 91 Denaturing Cycle Time=30s Annealing Temp=60 Annealing Time=30s Extension Temp=70 Extension Time=18mins Cycles=25 Final Extension Temp=0 Final Extension Time=0 4CTime=1 hr	{{{Denaturing Temp}}}	{{{Denaturing Time}}}
Buffer	{{{Buffer}}}	{{{Denaturing Cycle Temp}}}	{{{Denaturing Cycle Time}}}
Forward Primer	{{{PrimerF}}}	{{{Annealing Temp}}}	{{{Annealing Time}}}
Reverse Primer	{{{PrimerR}}}	{{{Extension Temp}}}	{{{Extension Time}}}
dNTPS	{{{dNTPs}}}	Cycles	{{{Cycles}}}
Template	{{{Template}}}	{{{Final Extension Temp}}}	{{{Final Extension Time}}}
Polymerase	{{{Polymerase}}}	4oC	{{{4CTime}}}
{{{Notes}}}