Notebook:Barry Canton/PCR boilerplate: Difference between revisions
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Latest revision as of 13:25, 18 February 2008
Not sure if this is going to be useful but we'll see.
Reaction Mix (μl) | Thermocycling | ||
---|---|---|---|
H2O | 39 | 95 | 1min |
Buffer | 5 Thermo Pol | 91 | 30s |
Forward Primer | 1.5 R0183rRNAF | 60 | 30s |
Reverse Primer | 1.5 rRNA-4RTHR1/rRNA-4RTHR2 | 70 | 18mins |
dNTPS | 2 | Cycles | 25 |
Template | 1 1/100 of template prep | 0 | 0 |
Polymerase | 2 Vent | 4oC | 1 hr |
First two reactions used rRNA-4 as template. Last two used fixed. |
Copy this text:
{{PCR reaction| Water= |Buffer= |PrimerF= |PrimerR= |dNTPs= |Template= |Polymerase= |Denaturing Temp= |Denaturing Time= |Denaturing Cycle Temp= |Denaturing Cycle Time= |Annealing Temp= |Annealing Time= |Extension Temp= |Extension Time= |Cycles= |Final Extension Temp= |Final Extension Time= |4CTime= |Notes= }}