OHSU:Keeping Frogs

...or use the toolbar with the icon that looks like a signature

Home        Projects        Team        Papers        Contact Us        Protocols        Collaborations        News        Reagent Requests        secret back door        Hoatlin Lab Twitter       

Comments from Jan's Lab (from Devo). I think that it would be helpful to discuss how you make the extracts so that everyone in Jan's understands when you need eggs, how many eggs you need, how they need to be collected, and how much advance warning you need from us about our spawning schedule, etc. It recently became obvious that not every one in Jan's lab is aware of how you use the eggs and what sort of constraints you have (e.g., the length of time it takes to make extracts, so you don't want eggs in the afternoon.) That said, many of us prefer to wait until after 12 noon to begin fertilizing eggs, so we would LOVE to get our hands on frogs that are giving in the afternoon. Comments from Maureen's Lab --Stacie 19:11, 26 February 2007 (EST) In order for us to use eggs they need to be spawned in 1X MMR (we are happy to provide 10X MMR any time for eggs, please let us know if you get low 4-5427). Extracts are best if eggs are spawned at 17 degrees and are relatively fresh (<3-4 hours old). In addition we find that preprimed frogs (48 hours to 2 weeks before HCG injections) are by far better producers of eggs. --Alexis 19:50, 26 February 2007 (EST) Email seems to work well to notify me (lachapel@ohsu.edu)/our lab (4-5427) when Christian lab is spawning. The earlier notice the better so I can plan time for extracts and not waste eggs. I will use Christian lab leftovers until crisis situation eases up. I'll check before taking frogs if we absolutely need eggs when no one is spawning. Questions? --Stacie 20:17, 26 February 2007 (EST)--What is the upper limit to the frog population per tank? So, does that mean you don't want the eggs laid overnight? We should be moving the frogs to 1XMMR first thing in the morning? Possible Solutions to Frog Shortage? --Stacie 20:19, 26 February 2007 (EST)--The resting tank dates are 1/23, 1/25, 2/6, 2/21 and the current use tank is a 12/4. Could we combine the 1/23 and 1/25 rest tanks? This will free up a tank for a new batch of 60 or so mature frogs that we can use to let the 1/23-25 tank rest longer. Action Items task/who will do it/when will it be done Shall we have a Frog useage Meeting? (Vote by adding you name below the desired response) Yes Devo Maureen Alexis Stacie No Possible Meeting Dates? Agenda Items for Meeting Estimate from each project on how many frogs (spawnings) are going to be needed Structure for the ideal way to share the eggs if possible Schedule/notification mechanism of when frogs are used for monitoring purposes Resting schedule to ensure frogs rest at least 3 months Prepriming discussion: is prepriming is effective or not? Do frogs need to rest even if they were preprimed/primed and did not produce eggs?