OhioMod2013:Introduction: Difference between revisions
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=Background= | =Background= | ||
[[DNA origami]] | [[DNA origami]]is a relatively recent technique first described in 2003 as a method of folding DNA into complex structures <cite>Rothemund</cite>. Since then there has been an effort to find practical applications for DNA origami, many of which involve targeted in vivo delivery of drug payloads <cite>Douglas</cite>, antigens <cite>Liu</cite>, and siRNA <cite> Lee</cite>. We believe however, that the strongest potential is for delivery of the DNA itself by incorporating the gene vector into the origami for cellular uptake. | ||
The viral method | |||
We first set out to develop a method of transfecting cells by a novel approach of encapsulating DNA origami with calcium phosphate to form biocompatible nanoparticles. This was a combinatorial process, | |||
Revision as of 12:01, 27 April 2013
Background
DNA origamiis a relatively recent technique first described in 2003 as a method of folding DNA into complex structures [1]. Since then there has been an effort to find practical applications for DNA origami, many of which involve targeted in vivo delivery of drug payloads [2], antigens [3], and siRNA [4]. We believe however, that the strongest potential is for delivery of the DNA itself by incorporating the gene vector into the origami for cellular uptake. The viral method We first set out to develop a method of transfecting cells by a novel approach of encapsulating DNA origami with calcium phosphate to form biocompatible nanoparticles. This was a combinatorial process,
Our Focus
To do this and that. See our Methods for more.
Our Goals
- Goal
- Goal
- Goal
- Gooooaal!
- Paul M Gruenbacher 12:42, 14 April 2013 (EDT):
