OhioMod2013:Results: Difference between revisions
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==Cellular Uptake== | ==Cellular Uptake== | ||
Use carbazole-based biscyanine fluorophores to measure intact DNA origami intracellularly. | |||
Also used flourescent tagged DNA origami. | |||
Also use flourescent tagged Calcium Phospahte nanoparticles. | |||
potential for confocal microscopy. | |||
Use chloroquinine to inhibit lysosomal fusion/acidification. Measure effect. | |||
Revision as of 10:14, 4 May 2013
Results
Nanoparticle Characterizatione
HPLC
Show A280 peaks, determine rate of DNA encapsulation vs free DNA.
Zetasizer
Determine size and zeta potential of particles.
TEM
Take direct measurements of ~100 particles. Determine consistency of size.
Dissolution
Perform titration of nanoparticles until dissolution of nanoparticles. Run gel for DNA origami vs. free DNA after dissolution. Is origami intact?
Serum Assay
Apply DNA encapsulated particles vs free DNA origami vs PEGylated particles to bovine serum. Measure intact origami after period of time.
Cytotoxicity
Perform MTT assay.
Cellular Uptake
Use carbazole-based biscyanine fluorophores to measure intact DNA origami intracellularly. Also used flourescent tagged DNA origami. Also use flourescent tagged Calcium Phospahte nanoparticles. potential for confocal microscopy.
Use chloroquinine to inhibit lysosomal fusion/acidification. Measure effect.
