OhioMod2013:Results

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==Cellular Uptake==
==Cellular Uptake==
 +
Use carbazole-based biscyanine fluorophores to measure intact DNA origami intracellularly.
 +
Also used flourescent tagged DNA origami.
 +
Also use flourescent tagged Calcium Phospahte nanoparticles.
 +
potential for confocal microscopy.
 +
 +
Use chloroquinine to inhibit lysosomal fusion/acidification. Measure effect.

Revision as of 12:14, 4 May 2013

Home        Introduction        Design        Methods        Results        Team        Internal       


Contents

Results

Nanoparticle Characterizatione

HPLC

Show A280 peaks, determine rate of DNA encapsulation vs free DNA.

Zetasizer

Determine size and zeta potential of particles.

TEM

Take direct measurements of ~100 particles. Determine consistency of size.

Dissolution

Perform titration of nanoparticles until dissolution of nanoparticles. Run gel for DNA origami vs. free DNA after dissolution. Is origami intact?

Serum Assay

Apply DNA encapsulated particles vs free DNA origami vs PEGylated particles to bovine serum. Measure intact origami after period of time.

Cytotoxicity

Perform MTT assay.

Cellular Uptake

Use carbazole-based biscyanine fluorophores to measure intact DNA origami intracellularly. Also used flourescent tagged DNA origami. Also use flourescent tagged Calcium Phospahte nanoparticles. potential for confocal microscopy.

Use chloroquinine to inhibit lysosomal fusion/acidification. Measure effect.

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