Oneill Lab:Chemicals: Difference between revisions
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Revision as of 13:13, 26 April 2007
Michael J. O'Neill Lab University of Connecticut Department of Molecular and Cell Biology
Common Lab Chemical Recipes
The recipes listed here can be found in the grey protocol binder
Church's Solution
Materials
- 20% SDS
- 0.5M EDTA pH=8.0
- 1M Na2HPO4
- diH2O
Procedure
- Mix 35ml SDS and 0.2ml EDTA
- Add 50ml Na2HPO4
- Bring final volume to 100ml
Note: SDS will precipitate at low temperatures. We have found keeping Church's Solution at 37°C may help in limiting instances of blot disease
Phosphate Buffered Saline
- Add 1 tablet per 100 ml diH2O
- Autoclave
20x SSC
Recipe to make 4L
- Dissolve 701.28g NaCl and 352.92g NaCitrate
- pH to 7.0
- Bring final volume to 4L
- Autoclave if necessary
20x TBE-modified
Recipe makes 2L
- 649.096g Tris
- 185.24g Boric Acid
- 37.968g EDTA
- Bring final volume to 2L
50x TAE
- 242g Tris
- 37.2g EDTA
- 57.1mL Glacial Acetic Acid
- Bring final volume to 1L with diH2O
20% SDS
200g SDS / 1L Wear mask and safety goggles! Heat to 68°C to dissolve