OpenWetWare:Getting started: Difference between revisions

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{{OpenWetWare:Getting started/Introduction}} <!-- Please do not touch this line! -->


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== Current Projects ==
David started here
'''Adenovirus Vaccines'''
OCW WAS HERE.
*[[Malaria]]
and so was kate-lol
*HPV
Cil tried this out today.
Peng too
Denil test editing
Editing id fun!
test line


Ta da!
'''Ubiquination'''
Sonia edited this! ha  <-  did I?  :o\


helloooooooooo...


me too..
== Protocols ==
<cite>somepaper anotherpaper andanotherpaper</cite>
*Adenovirus CaCl transfection
<biblio>
*Generating hexon mutants in E. coli
#somepaper pmid=16741115
*Generating fiber mutants in E. coli
#anotherpaper pmid=16699522
*Generating late gene expression mutants in 293 cells
#andanotherpaper pmid=16627746
*Purifying adenovius (banded stocks)
</biblio>
*Titration of viral stocks (calculate pfu)
*Calculate MOI
*Protein preps of infected 293 cells for westerns
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
*P32 label DNA


<!--Feel free to change, delete, or add text above this line. -->
==Materials==
|}
===Antibodies===
*[[anti-HA]]
*[[anti-Fiber]]
*[[anti-Late serum]]
*[[anti-NANP hybridoma supernatant]]
 
===Plasmids===
===Viruses===
===Cells===
 
== Links ==
[http://faculty.jhsph.edu/?F=Gary&L=Ketner Gary Ketner]
 
[http://www.jhsph.edu/PublicHealthNews/Magazine/ JHSPH Magazine]

Revision as of 06:24, 28 September 2006

Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA

Materials

Antibodies

Plasmids

Viruses

Cells

Links

Gary Ketner

JHSPH Magazine

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