OpenWetWare:Getting started: Difference between revisions

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[[LabName | <font face="trebuchet ms" style="color:#ffffff"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
[[LabName:Contact | <font face="trebuchet ms" style="color:#ffffff"> '''Contact''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
== Current Projects ==
[[LabName:Back Door | <font face="trebuchet ms" style="color:#ffffff"> '''Internal''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
'''Adenovirus Vaccines'''
[[LabName:Lab Members | <font face="trebuchet ms" style="color:#ffffff"> '''Lab Members''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
*[[Malaria]]
[[LabName:Publications | <font face="trebuchet ms" style="color:#ffffff"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
*HPV
[[LabName:Research | <font face="trebuchet ms" style="color:#ffffff"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
[[LabName:Talks | <font face="trebuchet ms" style="color:#ffffff"> '''Talks''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
'''Ubiquination'''
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== Protocols ==
*Adenovirus CaCl transfection
*Generating hexon mutants in E. coli
*Generating fiber mutants in E. coli
*Generating late gene expression mutants in 293 cells
*Purifying adenovius (banded stocks)
*Titration of viral stocks (calculate pfu)
*Calculate MOI
*Protein preps of infected 293 cells for westerns
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
*P32 label DNA
 
==Materials==
===Antibodies===
*[[anti-HA]]
*[[anti-Fiber]]
*[[anti-Late serum]]
*[[anti-NANP hybridoma supernatant]]
 
===Plasmids===
===Viruses===
===Cells===
 
== Links ==
[http://faculty.jhsph.edu/?F=Gary&L=Ketner Gary Ketner]
 
[http://www.jhsph.edu/PublicHealthNews/Magazine/ JHSPH Magazine]

Revision as of 13:54, 19 March 2014

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