OpenWetWare:Getting started: Difference between revisions

Revision as of 14:34, 10 August 2015

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   <a class="navbar-brand" href="http://openwetware.org/wiki/Biomod/2014/OhioMOD">OhioMOD</a>
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   <ul class="nav navbar-nav">
     <li ><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD">Home</a></li>
     <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/project">Background</a></li>

<li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/results">Project and Results</a></li>

       <a href="#" class="dropdown-toggle" data-toggle="dropdown">Materials and Methods <b class="caret"></b></a>
       <ul class="dropdown-menu">
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/protocols">Protocols</a></li>
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/equipment">Equipment</a></li>
         <li class="divider"></li>
         <li class="dropdown-header">Lab Notebook</li>
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/LabNotebook">Experiment Schedule</a></li>
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/experimentnotes">Experiment Notes</a></li>
       </ul>
     </li>

<li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/team">Team</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/sponsors">Acknowledgement</a></li> <li><a href="http://www.giveto.osu.edu/igive/OnlineGiving/fund_results.aspx?fund=314139" target="_blank">Give To OhioMOD</a></li>

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</style>  </head> <body> <div id="logo"> <img src="http://openwetware.org/images/e/ed/OHIOMODLOGO2.png" alt="OhioMOD"> <iframe src="//www.facebook.com/plugins/like.php?href=https%3A%2F%2Fwww.facebook.com%2FOhioMod&width&layout=standard&action=like&show_faces=true&share=true&height=80" scrolling="no" frameborder="0" style="border:none; overflow:hidden; height:80px;" allowTransparency="true"></iframe> </div> <div id="abstract"> <h1>Welcome to OhioMOD</h1> <p1>OhioMOD is Ohio State's undergraduate biomolecular design team. The team participates in the BIOMOD international competition each year at Harvard University. The 2014 project will use a folded DNA structure to control miRNA gene regulation in cancer.</p1> <h1>Abstract</h1> <p2>MicroRNAs (miRs) are short, non-coding RNAs that regulate gene expression and control essential cellular processes, such as cell cycle progression and apoptosis, through interactions with messenger RNA. Dysregulation of miR expression levels can promote tumorigenesis either by elevating oncogene or silencing tumor suppressor gene levels implicating miRs as an attractive therapeutic target. One therapeutic approach is to decrease levels of miRs that target tumor suppressor genes such as PTEN to ultimately promote apoptosis by complementary base pair antagonism via antisense DNA, but limitations exist in regard to antisense DNA cellular delivery. A previous study demonstrated that DNA nanostructures functionalized with small interfering RNA (siRNA) underwent efficient cellular entry and executed targeted gene suppression suggesting strong potential for DNA nanostructures as a nucleotide delivery vehicle. Here, we report the fabrication of a rod-like and a block-like DNA nanostructure incorporated with DNA overhangs capable of binding and sequestering miR-21, a PTEN targeting miR that is overexpressed in a variety of malignancies including chronic lymphocytic leukemia (CLL). Proper assembly was confirmed via gel electrophoresis and transmission electron microscopy. Incorporation and functionality of DNA overhangs complementary for miR-21 was confirmed via fluorescent gel imaging. In addition, DNA nanostructures were shown to enter the endolysosomal pathway as revealed via fluorescent microscopy. Furthermore, miR-21 complementary DNA overhang functionalized structures induced marked decreases in OSU-CLL cell viability relative to scrambled control overhang nanostructures as shown via fluorescent microscopy. Future work will directly investigate expression levels of PTEN mRNA and protein using quantitative real time PCR and immunoblot analysis respectively. Our current findings suggest that the complementary miR-21 DNA overhang functionalized DNA nanostructures effectively sequester miR-21 to induce cytotoxicity in OSU-CLL cells. Our results represent a promising antisense DNA delivery therapeutic approach in a CLL leukemia model with implications to a variety of human malignancies.</p2> <h1>Project Video</h1> <br> </div> <div id="video"> <center> <iframe width="660" height="415" src="//www.youtube.com/embed/OzRtxW4YK2c" frameborder="0" allowfullscreen></iframe> </center>

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<td id="email">Contact us at <a href="mailto:theohiomod@gmail.com"><font color="white">theohiomod@gmail.com</a></td>

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OpenWetWare:Getting started: Difference between revisions

Revision as of 14:34, 10 August 2015

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@@ Line 1: / Line 1: @@
-{{Navbar}}
+{{OhioMOD2014}}
-{|width="712px"
+<html>
-|
+<head>
-{{OpenWetWare:Getting started/Introduction}} <!-- Please do not touch this line! -->
+<!--css stuff begins here-->
+<style>
+center {padding-bottom:50px;}
+/*Aligning the Logo*/
+#logo {
+  width: 700px ;
+  margin-left: auto ;
+  margin-right: auto ;
+}
-<!-- Feel free to change, delete, or add text below this line. -->
+/*Aligning the Video*/
-David started here
+#video {
-OCW WAS HERE.
+  width: 700px ;
-and so was kate-lol
+  margin-left: auto ;
-Cil tried this out today.
+  margin-right: auto ;
-Peng too
+}
-Denil test editing
-Testing.
-Scott is here
-=== Niftericious ===
+/*Aligning the Abstract*/
-   indenting creates a text box
+#abstract {
-Editing is fun!
+  width: 700px ;
-test line
+   margin-left: auto ;
+  margin-right: auto ;
+}
-Ta da!
+/*fixed icon code start*/
-Sonia edited this! ha  <-  did I?  :o\
+#fixedbutton {
+    position: fixed;
+    bottom: 53px;
+    right: 10px; }
+/*fixed icon code end*/
-  WOOOOOOOOOOOOOOO
+</style>
+<!--css stuff ends here-->
+</head>
+<body>
+<div id="logo">
+<img src="http://openwetware.org/images/e/ed/OHIOMODLOGO2.png" alt="OhioMOD">
+<iframe src="//www.facebook.com/plugins/like.php?href=https%3A%2F%2Fwww.facebook.com%2FOhioMod&amp;width&amp;layout=standard&amp;action=like&amp;show_faces=true&amp;share=true&amp;height=80" scrolling="no" frameborder="0" style="border:none; overflow:hidden; height:80px;" allowTransparency="true"></iframe>
+</div>
+<div id="abstract">
+<h1>Welcome to OhioMOD</h1>
+<p1>OhioMOD is Ohio State's undergraduate biomolecular design team.  The team participates in the BIOMOD international competition each year at Harvard University.  The 2014 project will use a folded DNA structure to control miRNA gene regulation in cancer.</p1>
+<h1>Abstract</h1>
+<p2>MicroRNAs (miRs) are short, non-coding RNAs that regulate gene expression and control essential cellular processes, such as cell cycle progression and apoptosis, through interactions with messenger RNA. Dysregulation of miR expression levels can promote tumorigenesis either by elevating oncogene or silencing tumor suppressor gene levels implicating miRs as an attractive therapeutic target. One therapeutic approach is to decrease levels of miRs that target tumor suppressor genes such as PTEN to ultimately promote apoptosis by complementary base pair antagonism via antisense DNA, but limitations exist in regard to antisense DNA cellular delivery. A previous study demonstrated that DNA nanostructures functionalized with small interfering RNA (siRNA) underwent efficient cellular entry and executed targeted gene suppression suggesting strong potential for DNA nanostructures as a nucleotide delivery vehicle. Here, we report the fabrication of a rod-like
+and a block-like DNA nanostructure incorporated with DNA overhangs capable of binding and sequestering miR-21, a PTEN targeting miR that is overexpressed in a variety of malignancies including chronic lymphocytic leukemia (CLL). Proper assembly was confirmed via gel electrophoresis and transmission electron microscopy. Incorporation and functionality of DNA overhangs complementary for miR-21 was confirmed via fluorescent gel imaging. In addition, DNA nanostructures were shown to enter the endolysosomal pathway as revealed via fluorescent microscopy. Furthermore, miR-21 complementary DNA overhang functionalized structures induced marked decreases in OSU-CLL cell viability relative to scrambled control overhang nanostructures as shown via fluorescent microscopy. Future work will directly investigate expression levels of PTEN mRNA and protein using quantitative real time PCR and immunoblot analysis respectively. Our current findings suggest that the complementary miR-21 DNA overhang functionalized DNA nanostructures effectively sequester miR-21 to induce cytotoxicity in OSU-CLL cells. Our results represent a promising antisense DNA delivery therapeutic approach in a CLL leukemia model with implications to a variety of human malignancies.</p2>
+<h1>Project Video</h1>
+<br>
+</div>
+<div id="video">
+<center>
+<iframe width="660" height="415" src="//www.youtube.com/embed/OzRtxW4YK2c" frameborder="0" allowfullscreen></iframe>
+</center>
-namwoo kim
+<A HREF="#top"><img src="http://openwetware.org/images/4/46/Arrow_up.png" id="fixedbutton"height="40" width="40"></A>
+</br><a href="http://openwetware.org/index.php?title=Biomod/2014/OhioMOD&action=edit">Edit Main Page</a>
+<a href="http://info.flagcounter.com/4FJ3"><img src="http://s06.flagcounter.com/count/4FJ3/bg_FFFFFF/txt_000000/border_CCCCCC/columns_8/maxflags_12/viewers_3/labels_1/pageviews_1/flags_0/" alt="Flag Counter" border="0"></a>
+</div>
-helloooooooooo..
+</body>
+</html>
-me too..
+{{OSUFooter}}
-<cite>somepaper anotherpaper andanotherpaper</cite>
+{{OpenWetWare:Getting started/Introduction}} <!-- PLEASE DO NOT MAKE EDITS TO THIS PAGE! If you would like to make test edits, please refer here: http://openwetware.org/wiki/Sandbox -->
-<biblio>
-#somepaper pmid=16741115
-#anotherpaper pmid=16699522
-#andanotherpaper pmid=16627746
-</biblio>
-hello!
-<!--Feel free to change, delete, or add text above this line. -->
-Ένα παράδειγμα για το πως μπορεί να χρησιμοποιηθεί το wiki στη διδασκαλίας της Βιολογίας
-Hello!!!
-   This is a test.