OriC/ter ratio determination
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This protocol describes how one can determine the ration of the origin of termination oriC and the terminus of replication ter in Escherichia coli. That is for example important for cell cycle analysis. To get the ratio one could use Southern Blot and quantification of the bands. However, this protocol describes the use of qPCR which is a bit faster.
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Biological resources e.g. cell lines, buffers (link to a method for making them), enzymes, chemicals, kits, etc.
Any equipment used to perform the protocol (link to a method for using them).
A step by step guide to the experimental procedure.
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Referenced from the main protocol, a more thorough explanation of particularly important steps in the protocol.
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