PBS: Difference between revisions
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# Add H<sub>2</sub>O to 1L | # Add H<sub>2</sub>O to 1L | ||
# Autoclave for 20 minutes on liquid cycle. Store at RT. | # Autoclave for 20 minutes on liquid cycle. Store at RT. | ||
*'''[[PBST]]''': For some applications (washing) one adds Tween 20 PBS which makes [[PBST]] | |||
**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%. | |||
==Other Links== | ==Other Links== | ||
Revision as of 02:49, 12 December 2008
Purpose
PBS stands for phosphate buffered saline and is often used because it is isotonic to cells.
Recipe
1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4
To make 10x PBS,
- Combine the following:
- 80 g NaCl
- 2 g KCl
- 14.4 g Na2HPO4
- 2.4 g KH2PO4
- 800 mL distilled H2O
- Adjust pH to 7.4 with HCl
- Add H2O to 1L
- Autoclave for 20 minutes on liquid cycle. Store at RT.
- PBST: For some applications (washing) one adds Tween 20 PBS which makes PBST
- Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
