PBS
From OpenWetWare
(Difference between revisions)
(→Buffers) |
|||
| Line 19: | Line 19: | ||
4. Autoclave for 20 minutes on liquid cycle. Store at RT. | 4. Autoclave for 20 minutes on liquid cycle. Store at RT. | ||
| + | |||
| + | ==Other Links== | ||
| + | [[Silver: 10x PBS]] | ||
Revision as of 15:45, 23 March 2006
1. Combine the following:
80 g NaCl
2 g KCl
14.4 g Na2HPO4
2.4 g KH2PO4
800 mL distilled H2O
2. Adjust pH to 7.4 with HCl
3. Add H2O to 1L
4. Autoclave for 20 minutes on liquid cycle. Store at RT.


