PBS

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (10:22, 22 April 2009) (view source)
(Recipe: added dibasic heptahydrate)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
1. Combine the following:
+
==Purpose==
 +
PBS stands for phosphate buffered saline and is often used because it is isotonic to cells.
-
80 g NaCl
+
==Recipe==
 +
1x PBS is 137 mM NaCl, 12 mM Phosphate, 2.7 mM KCl, pH 7.4
-
2 g KCl
+
To make 10x PBS,
 +
# Combine the following:
 +
#* 80 g NaCl
 +
#* 2 g KCl
 +
#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub> (dibasic anhydrous) OR 18.1 g Na<sub>2</sub>HPO<sub>4</sub> · 2H<sub>2</sub>O (dibasic dihydrate) OR 27.2 g Na<sub>2</sub>HPO<sub>4</sub> · 7H<sub>2</sub>O (dibasic heptahydrate)
 +
#* 2.4 g KH<sub>2</sub>PO<sub>4</sub> (monobasic anhydrous)
 +
#* 800 mL distilled H<sub>2</sub>O
 +
# Adjust pH to 7.4 with HCl
 +
# Add H<sub>2</sub>O to 1L
 +
# Autoclave for 20 minutes on liquid cycle. Store at RT.
-
14.4 g Na2HPO4
 
-
2.4 g KH2PO4
+
*'''[[PBST]]''': For some applications (washing) one adds Tween 20 PBS which makes [[PBST]]
 +
**Usual concentrations of Tween 20 in PBST are 0.1 or 0.05%.
 +
**Usual concentration of Triton X-100 in TBST is 0.1-0.3%.
-
800 mL distilled H2O
+
==Other Links==
 +
* [[Silver: 10x PBS]]
 +
* [http://en.wikipedia.org/wiki/Phosphate_buffered_saline wikipedia article]
-
2. Adjust pH to 7.4 with HCl
+
[[Category:Material]] [[Category:Buffers]]
-
 
+
-
3. Add H2O to 1L
+
-
 
+
-
4. Autoclave for 20 minutes on liquid cycle. Store at RT.
+

Current revision

Purpose

PBS stands for phosphate buffered saline and is often used because it is isotonic to cells.

Recipe

1x PBS is 137 mM NaCl, 12 mM Phosphate, 2.7 mM KCl, pH 7.4

To make 10x PBS,

  1. Combine the following:
    • 80 g NaCl
    • 2 g KCl
    • 14.4 g Na2HPO4 (dibasic anhydrous) OR 18.1 g Na2HPO4 · 2H2O (dibasic dihydrate) OR 27.2 g Na2HPO4 · 7H2O (dibasic heptahydrate)
    • 2.4 g KH2PO4 (monobasic anhydrous)
    • 800 mL distilled H2O
  2. Adjust pH to 7.4 with HCl
  3. Add H2O to 1L
  4. Autoclave for 20 minutes on liquid cycle. Store at RT.


  • PBST: For some applications (washing) one adds Tween 20 PBS which makes PBST
    • Usual concentrations of Tween 20 in PBST are 0.1 or 0.05%.
    • Usual concentration of Triton X-100 in TBST is 0.1-0.3%.

Other Links

Personal tools