PBS

Purpose

PBS stands for phosphate buffered saline and is often used because it is isotonic to cells.

Recipe

1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4

To make 10x PBS,

1. Combine the following:
   • 80 g NaCl
   • 2 g KCl
   • 14.4 g Na₂HPO₄
   • 2.4 g KH₂PO₄
   • 800 mL distilled H₂O
2. Adjust pH to 7.4 with HCl
3. Add H₂O to 1L
4. Autoclave for 20 minutes on liquid cycle. Store at RT.

Other Links

• Silver: 10x PBS
• wikipedia article and