PBST: Difference between revisions
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== Purpose == | == Purpose == | ||
* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes | * PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment. | ||
* PBST is used in [[protein blots]] for washing of membrane labelled with antibody. | * PBST is used in [[protein blots]] for washing of membrane labelled with antibody. | ||
Latest revision as of 18:42, 23 December 2012
PBS stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both).
Purpose
- PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
- PBST is used in protein blots for washing of membrane labelled with antibody.
Recipe
0.1% (v/v) Tween 20 in 1x PBS
examples
- 100µl in 100ml 1x PBS
- 1ml in 1L 1xPBS
