PBST

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Line 1: Line 1:
==Purpose==
==Purpose==
-
* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or TritonX100 makes it PBST (people use the name PBST for both).  
+
* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST for both).  
*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.

Revision as of 09:41, 12 January 2009

Purpose

  • PBS stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST for both).
  • PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.

Recipe

Personal tools