PBST
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Current revision (21:42, 23 December 2012) (view source) (→Purpose) |
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| - | + | [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both). | |
| - | + | ||
| - | == | + | == Purpose == |
| - | * | + | * PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment. |
| + | * PBST is used in [[protein blots]] for washing of membrane labelled with antibody. | ||
| + | |||
| + | == Recipe == | ||
| + | 0.1% (v/v) Tween 20 in 1x [[PBS]] | ||
| + | |||
| + | examples | ||
| + | * 100µl in 100ml 1x PBS | ||
| + | * 1ml in 1L 1xPBS | ||
[[Category:Material]] [[Category:Buffers]] | [[Category:Material]] [[Category:Buffers]] | ||
Current revision
PBS stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both).
Purpose
- PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
- PBST is used in protein blots for washing of membrane labelled with antibody.
Recipe
0.1% (v/v) Tween 20 in 1x PBS
examples
- 100µl in 100ml 1x PBS
- 1ml in 1L 1xPBS
