PCR Primers for Transgenic Mice: Difference between revisions
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Tet-Responsive Lines (tetO) | Tet-Responsive Lines (tetO) | ||
Transgene: tetO-Lac Z | *Transgene: tetO-Lac Z | ||
5´ primer: GGC GTG TAC GGT GGG AGG | *5´ primer: GGC GTG TAC GGT GGG AGG | ||
3´ primer: GTT GGG AAG GGC GAT CGG | *3´ primer: GTT GGG AAG GGC GAT CGG | ||
PCR Fragment Size: 421 bp | *PCR Fragment Size: 421 bp | ||
Transgene: tetO-G-alpha | *Transgene: tetO-G-alpha 12 | ||
5´ primer: GCA | *5´ primer: CGA GAG CGG CAA GTC CAC | ||
*3´ primer: GCA GGC TCA GTC GGC ATG TAC | |||
*PCR Fragment Size: 245 bp | |||
3´ primer: TCT GTC GGC ATG TAC TCC TC | *Transgene: tetO-G-alpha i2 | ||
*5´ primer: GCA GAT CGA CTT TGC TGA CC | |||
*3´ primer: TCT GTC GGC ATG TAC TCC TC | |||
*PCR Fragment Size: 240 bp | |||
PCR Fragment Size: | *Transgene: tetO-G-alpha q | ||
*5´ primer: TTG ACG TCC CCG ACT ACG CG | |||
*3´ primer: CTT TGG CCC CCT ACA TCG AC | |||
*PCR Fragment Size: 258 bp | |||
5´ primer: | *Transgene: tetO-Ro1/Ro2 | ||
3´ primer: | *5´ primer (Go148): GTT GGG AAG GGC GAT CGG | ||
*3´ primer (Go147): GGC GTC TAC GGT GGG AGG | |||
*PCR Fragment Size: 178 bp | |||
Transactivator Lines | |||
*Transgene: tTA | |||
Transgene: tTA | *5´ primer: GCT GCT TAA TGA GGT CGG | ||
5´ primer: GCT GCT TAA TGA GGT CGG | *3´ primer: CTC TGC ACC TTG GTG ATC | ||
3´ primer: CTC TGC ACC TTG GTG ATC | *PCR Fragment Size: 507 bp | ||
PCR Fragment Size: 507 bp | |||
Transgene: MH-tTA | *Transgene: MH-tTA | ||
5´ primer: TCA GAC CGA GAT TTC TTC ATC CC | *5´ primer: TCA GAC CGA GAT TTC TTC ATC CC | ||
3´ primer: CTC TGC ACC TTG GTG ATC | *3´ primer: CTC TGC ACC TTG GTG ATC | ||
PCR Fragment Size: ~1,000 bp | *PCR Fragment Size: ~1,000 bp | ||
Transgene: MMTV-tTA | *Transgene: MMTV-tTA | ||
5´ primer: CTG ATC TGA GCT CTG AGT G | *5´ primer: CTG ATC TGA GCT CTG AGT G | ||
3´ primer: CTC TGC ACC TTG GTG ATC | *3´ primer: CTC TGC ACC TTG GTG ATC | ||
PCR Fragment Size: 595 bp | *PCR Fragment Size: 595 bp | ||
'''Protocols for genotyping the following mice can be found on the Protocol's Page:''' | '''Protocols for genotyping the following mice can be found on the Protocol's Page:''' | ||
Latest revision as of 14:13, 29 June 2009
Conklin Lab Transgenic Mice
The Conklin Lab maintains several transgenic lines that utilize the tetracyclinetransactivator system. These lines will be made available on a collaborative basis and as our limited resources allow. (Jackson Lab should have manyof these lines soon).
Genotyping mice
Mice can be genotyped by PCR. After digesting a small piece of mousetail with proteinase K, a small alliquot of sample which contains genomic DNA is analyzed by PCR using transgene specific primers. Full protocol available.
All oligonucleotide sequences are shown as they should be ordered.
Tet-Responsive Lines (tetO)
- Transgene: tetO-Lac Z
*5´ primer: GGC GTG TAC GGT GGG AGG *3´ primer: GTT GGG AAG GGC GAT CGG *PCR Fragment Size: 421 bp
- Transgene: tetO-G-alpha 12
*5´ primer: CGA GAG CGG CAA GTC CAC *3´ primer: GCA GGC TCA GTC GGC ATG TAC *PCR Fragment Size: 245 bp
- Transgene: tetO-G-alpha i2
*5´ primer: GCA GAT CGA CTT TGC TGA CC *3´ primer: TCT GTC GGC ATG TAC TCC TC *PCR Fragment Size: 240 bp
- Transgene: tetO-G-alpha q
*5´ primer: TTG ACG TCC CCG ACT ACG CG *3´ primer: CTT TGG CCC CCT ACA TCG AC *PCR Fragment Size: 258 bp
- Transgene: tetO-Ro1/Ro2
*5´ primer (Go148): GTT GGG AAG GGC GAT CGG *3´ primer (Go147): GGC GTC TAC GGT GGG AGG *PCR Fragment Size: 178 bp
Transactivator Lines
- Transgene: tTA
*5´ primer: GCT GCT TAA TGA GGT CGG *3´ primer: CTC TGC ACC TTG GTG ATC *PCR Fragment Size: 507 bp
- Transgene: MH-tTA
*5´ primer: TCA GAC CGA GAT TTC TTC ATC CC *3´ primer: CTC TGC ACC TTG GTG ATC *PCR Fragment Size: ~1,000 bp
- Transgene: MMTV-tTA
*5´ primer: CTG ATC TGA GCT CTG AGT G *3´ primer: CTC TGC ACC TTG GTG ATC *PCR Fragment Size: 595 bp
Protocols for genotyping the following mice can be found on the Protocol's Page:
TetO-Rs1 mice: Protocol to Genotype Rs1 Mice [1]
ColI(2.3)-tTA mice Protocol to Genotype ColI [2] (2.3)-tTA Mice: R. 20080528
NKX2-5-EmGFP (RP11-88L12 NKX2-5 EmGFP reporter mice) Protocol to Genotype NKX2-5 BAC Mice [3]
