PCR Primers for Transgenic Mice

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'''All oligonucleotide sequences are shown as they should be ordered.'''
'''All oligonucleotide sequences are shown as they should be ordered.'''
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[[Image:tet-O.tiff]]
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Tet-Responsive Lines (tetO)
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[[Image:transactivator lines.tiff]]
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Transgene: tetO-Lac Z
 +
5´ primer: GGC GTG TAC GGT GGG AGG
 +
3´ primer: GTT GGG AAG GGC GAT CGG
 +
PCR Fragment Size: 421 bp
 +
 +
Transgene: tetO-G-alpha 12
 +
5´ primer: CGA GAG CGG CAA GTC CAC
 +
3´ primer: GCA GGC TCA GTC GGC ATG TAC
 +
PCR Fragment Size: 245 bp
 +
 
 +
Transgene: tetO-G-alpha i2
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5´ primer: GCA GAT CGA CTT TGC TGA CC
 +
 
 +
3´ primer: TCT GTC GGC ATG TAC TCC TC
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PCR Fragment Size: 240 bp
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Transgene: tetO-G-alpha q
 +
 
 +
5´ primer: TTG ACG TCC CCG ACT ACG CG
 +
3´ primer: CTT TGG CCC CCT ACA TCG AC
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 +
PCR Fragment Size: 258 bp
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 +
 
 +
Transgene: tetO-Ro1/Ro2
 +
5´ primer (Go148): GTT GGG AAG GGC GAT CGG
 +
3´ primer (Go147): GGC GTC TAC GGT GGG AGG
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PCR Fragment Size: 178 bp
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 +
Transactivator Lines
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Transgene: tTA
 +
5´ primer: GCT GCT TAA TGA GGT CGG
 +
3´ primer: CTC TGC ACC TTG GTG ATC
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PCR Fragment Size: 507 bp
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Transgene: MH-tTA
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5´ primer: TCA GAC CGA GAT TTC TTC ATC CC
 +
3´ primer: CTC TGC ACC TTG GTG ATC
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PCR Fragment Size: ~1,000 bp
 +
 
 +
Transgene: MMTV-tTA
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5´ primer: CTG ATC TGA GCT CTG AGT G
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3´ primer: CTC TGC ACC TTG GTG ATC
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PCR Fragment Size: 595 bp
'''Protocols for genotyping the following mice can be found on the Protocol's Page:'''
'''Protocols for genotyping the following mice can be found on the Protocol's Page:'''

Revision as of 17:08, 29 June 2009

Conklin Lab Transgenic Mice

The Conklin Lab maintains several transgenic lines that utilize the tetracyclinetransactivator system. These lines will be made available on a collaborative basis and as our limited resources allow. (Jackson Lab should have manyof these lines soon).

Genotyping mice

Mice can be genotyped by PCR. After digesting a small piece of mousetail with proteinase K, a small alliquot of sample which contains genomic DNA is analyzed by PCR using transgene specific primers. Full protocol available.

All oligonucleotide sequences are shown as they should be ordered.

Tet-Responsive Lines (tetO)

Transgene: tetO-Lac Z 5´ primer: GGC GTG TAC GGT GGG AGG 3´ primer: GTT GGG AAG GGC GAT CGG PCR Fragment Size: 421 bp

Transgene: tetO-G-alpha 12 5´ primer: CGA GAG CGG CAA GTC CAC 3´ primer: GCA GGC TCA GTC GGC ATG TAC PCR Fragment Size: 245 bp

Transgene: tetO-G-alpha i2 5´ primer: GCA GAT CGA CTT TGC TGA CC

3´ primer: TCT GTC GGC ATG TAC TCC TC

PCR Fragment Size: 240 bp

Transgene: tetO-G-alpha q

5´ primer: TTG ACG TCC CCG ACT ACG CG 3´ primer: CTT TGG CCC CCT ACA TCG AC

PCR Fragment Size: 258 bp


Transgene: tetO-Ro1/Ro2 5´ primer (Go148): GTT GGG AAG GGC GAT CGG 3´ primer (Go147): GGC GTC TAC GGT GGG AGG PCR Fragment Size: 178 bp

Transactivator Lines Transgene: tTA 5´ primer: GCT GCT TAA TGA GGT CGG 3´ primer: CTC TGC ACC TTG GTG ATC PCR Fragment Size: 507 bp

Transgene: MH-tTA 5´ primer: TCA GAC CGA GAT TTC TTC ATC CC 3´ primer: CTC TGC ACC TTG GTG ATC PCR Fragment Size: ~1,000 bp

Transgene: MMTV-tTA 5´ primer: CTG ATC TGA GCT CTG AGT G 3´ primer: CTC TGC ACC TTG GTG ATC PCR Fragment Size: 595 bp

Protocols for genotyping the following mice can be found on the Protocol's Page:

TetO-Rs1 mice: Protocol to Genotype Rs1 Mice [1]


ColI(2.3)-tTA mice Protocol to Genotype ColI [2] (2.3)-tTA Mice: R. 20080528


NKX2-5-EmGFP (RP11-88L12 NKX2-5 EmGFP reporter mice) Protocol to Genotype NKX2-5 BAC Mice [3]

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