PCR inhibitors: Difference between revisions
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Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present. | Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present. | ||
== Inhibitors from the purification process == | |||
== Inhibitors from the source tissue == | |||
== See also == | |||
[[Category:PCR]] [[Category:DNA]] [[Category:In vitro]] [[Category:Chemicals]] | [[Category:PCR]] [[Category:DNA]] [[Category:In vitro]] [[Category:Chemicals]] |
Revision as of 02:53, 7 July 2010
Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.