PCR inhibitors

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Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.
Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.
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== Inhibitors from the purification process ==
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== Inhibitors from the source tissue ==
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== See also ==
[[Category:PCR]] [[Category:DNA]] [[Category:In vitro]] [[Category:Chemicals]]
[[Category:PCR]] [[Category:DNA]] [[Category:In vitro]] [[Category:Chemicals]]

Revision as of 05:53, 7 July 2010

Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.

Inhibitors from the purification process

Inhibitors from the source tissue

See also

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