PCR inhibitors

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(illustration)
(Inhibitors from the purification process)
Line 4: Line 4:
== Inhibitors from the purification process ==
== Inhibitors from the purification process ==
 +
 +
* alcohols like '''ethanol''' and '''2-propanol''' (isopropanol) from DNA template precipitation ([http://www.qiagen.com/literature/qiagennews/0497/974compl.pdf Qiagen test of sequencing inhibition by Welters et al. 1997])
 +
* organic solvents like '''phenol''' from phenol/chloroform purification (PMID 8136148)
 +
* salts like '''KCl''', '''NaCl''' from precipitation
 +
* detergents like '''SDS''',.. from membrane lysis (PMID 2223070)
== Inhibitors from the source tissue ==
== Inhibitors from the source tissue ==

Revision as of 06:23, 7 July 2010

Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.

Contents

Inhibitors from the purification process

Inhibitors from the source tissue

Template inhibition

See also

Personal tools