PCR inhibitors: Difference between revisions
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== Inhibitors from the purification process == | == Inhibitors from the purification process == | ||
* alcohols like '''ethanol''' and '''2-propanol''' (isopropanol) from DNA template precipitation ([http://www.qiagen.com/literature/qiagennews/0497/974compl.pdf Qiagen test of sequencing inhibition by Welters et al. 1997]) | |||
* organic solvents like '''phenol''' from phenol/chloroform purification (PMID 8136148) | |||
* salts like '''KCl''', '''NaCl''' from precipitation | |||
* detergents like '''SDS''',.. from membrane lysis (PMID 2223070) | |||
== Inhibitors from the source tissue == | == Inhibitors from the source tissue == | ||
Revision as of 04:23, 7 July 2010
Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.
Inhibitors from the purification process
- alcohols like ethanol and 2-propanol (isopropanol) from DNA template precipitation (Qiagen test of sequencing inhibition by Welters et al. 1997)
- organic solvents like phenol from phenol/chloroform purification (PMID 8136148)
- salts like KCl, NaCl from precipitation
- detergents like SDS,.. from membrane lysis (PMID 2223070)
