PCR inhibitors

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(Template inhibition)
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== Template inhibition ==
== Template inhibition ==
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Since inhibitors often enter the PCR reaction via the template preparation, decreasing template volume may improve PCR results indirectly by reducing the quantity of inhibitors.
== See also ==
== See also ==

Revision as of 08:14, 7 July 2010

Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.

Contents

Inhibitors from the purification process

Inhibitors from the source tissue

  • components of blood: heme, hemoglobin, lactoferrin, immunoglobin G (IgG)
  • liver, digestive tract, feces: bile salts, polysaccharides
  • connective tissue, skin: collagen, melanin
  • urine: urea

See Rådström et al. 2004 (PMID 14764939) for a review on how to avoid inhibition from tissue components.

Template inhibition

Since inhibitors often enter the PCR reaction via the template preparation, decreasing template volume may improve PCR results indirectly by reducing the quantity of inhibitors.

See also

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