PHYC452Biophysics:Schedule/quiz3: Difference between revisions
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(New page: Quiz 3 Topics This quiz will cover the Beer-Lambert Law, absorbance measurements for protein and nucleic acid concentration measurements, and the laser classes for visible, CW lasers. S...) |
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*The power ranges and safety considerations for visible, CW lasers for class I,II,IIa,IIIa,IIIb,IV lasers. | *The power ranges and safety considerations for visible, CW lasers for class I,II,IIa,IIIa,IIIb,IV lasers. | ||
**See [http://en.wikipedia.org/wiki/Laser_Safety Laser Safety] and consider only the old system (not revised). | **See [http://en.wikipedia.org/wiki/Laser_Safety Laser Safety] and consider only the old system (not revised). | ||
Hey does any one know the answers to thee points. I don't know where to find them online. | |||
#The wavelengths used for protein, vs nucleic acids. | |||
#*280 for protein. 260 for Nucleic Acid | |||
#Absorbance at these wavelengths is primarly from ring structures | |||
#The three amino acids that contribute most to protein absorbance | |||
*they have the ring structure and are phenylalanine, tryptophan, and tyrosine. | |||
Latest revision as of 15:06, 23 April 2008
Quiz 3 Topics
This quiz will cover the Beer-Lambert Law, absorbance measurements for protein and nucleic acid concentration measurements, and the laser classes for visible, CW lasers.
Students should know:
- The equation for the Beer-Lambert law
- The definition of absorbance and transmittance
- The units of molar extinction coefficient
- How to convert from a absorption cross section to molar extinction coefficient
- The wavelengths used for protein, vs nucleic acids.
- Absorbance at these wavelengths is primarly from ring structures
- The three amino acids that contribute most to protein absorbance
- The power ranges and safety considerations for visible, CW lasers for class I,II,IIa,IIIa,IIIb,IV lasers.
- See Laser Safety and consider only the old system (not revised).
Hey does any one know the answers to thee points. I don't know where to find them online.
- The wavelengths used for protein, vs nucleic acids.
- 280 for protein. 260 for Nucleic Acid
- Absorbance at these wavelengths is primarly from ring structures
- The three amino acids that contribute most to protein absorbance
- they have the ring structure and are phenylalanine, tryptophan, and tyrosine.
