PHYC500/2007F:Interactions: Difference between revisions
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If you use a chemical that can cross-link biological components together, what controls could you do to see whether that cross-linking changes the spatial distribution of the components? - JLT | If you use a chemical that can cross-link biological components together, what controls could you do to see whether that cross-linking changes the spatial distribution of the components? - JLT | ||
I think the reason why we can't look at living things is the fact that we have to put the samples in a vacuum. The EM uses an electron beam to illuminate and create an image of a cell. Hence, molecules in air would scatter | I think the reason why we can't look at living things is the fact that we have to put the samples in a vacuum. The EM uses an electron beam to illuminate and create an image of a cell. Hence, molecules in air would scatter the electrons :( -KS | ||
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Revision as of 20:41, 28 August 2007
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Test message 1
Test message--Skoch3 17:13, 8 August 2007 (EDT)
Electron microscopy requires that cells be "fixed" (chemically cross-linked). Why? Why can't we look at living cells in an electron microscope?
If you use a chemical that can cross-link biological components together, what controls could you do to see whether that cross-linking changes the spatial distribution of the components? - JLT
I think the reason why we can't look at living things is the fact that we have to put the samples in a vacuum. The EM uses an electron beam to illuminate and create an image of a cell. Hence, molecules in air would scatter the electrons :( -KS
