PIPES
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piperazine-N,Nā-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757 | piperazine-N,Nā-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757 | ||
| - | A Good buffer <cite>Good66</cite><cite>Blanchard84</cite>. | + | A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. |
* pK<sub>a</sub> at 25C of 6.8 (a second one at pH 3 is not of interest) | * pK<sub>a</sub> at 25C of 6.8 (a second one at pH 3 is not of interest) | ||
* usable buffering range of 6.1 to 7.5 | * usable buffering range of 6.1 to 7.5 | ||
* molecular weight 302.4 | * molecular weight 302.4 | ||
| - | * ΔpK<sub>a</sub>/ΔT = .0085 | + | * ΔpK<sub>a</sub>/ΔT = -.0085 |
Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized. | Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized. | ||
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<biblio> | <biblio> | ||
# Good66 pmid=5942950 | # Good66 pmid=5942950 | ||
| + | # Good74 pmid=4206745 | ||
# Blanchard84 pmid=6717292 | # Blanchard84 pmid=6717292 | ||
</biblio> | </biblio> | ||
| + | |||
| + | [[Category:Material]] and [[Category:Buffers]] | ||
Current revision
piperazine-N,Nā-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757
- pKa at 25C of 6.8 (a second one at pH 3 is not of interest)
- usable buffering range of 6.1 to 7.5
- molecular weight 302.4
- ΔpKa/ΔT = -.0085
Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized.
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. . pmid:5942950.
- Good NE and Izawa S. . pmid:4206745.
- Blanchard JS. . pmid:6717292.
