PIPES: Difference between revisions
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piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757 | piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757 | ||
A Good buffer <cite>Good66</cite><cite>Blanchard84</cite>. | A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. | ||
* pK<sub>a</sub> at 25C of 6.8 (a second one at pH 3 is not of interest) | * pK<sub>a</sub> at 25C of 6.8 (a second one at pH 3 is not of interest) | ||
| Line 12: | Line 12: | ||
<biblio> | <biblio> | ||
# Good66 pmid=5942950 | # Good66 pmid=5942950 | ||
# Good74 pmid=4206745 | |||
# Blanchard84 pmid=6717292 | # Blanchard84 pmid=6717292 | ||
</biblio> | </biblio> | ||
Revision as of 15:09, 14 April 2006
piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757
- pKa at 25C of 6.8 (a second one at pH 3 is not of interest)
- usable buffering range of 6.1 to 7.5
- molecular weight 302.4
- ΔpKa/ΔT = -.0085
Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized.
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. DOI:10.1021/bi00866a011 |
- Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53-68. DOI:10.1016/0076-6879(72)24054-x |
- Blanchard JS. Buffers for enzymes. Methods Enzymol. 1984;104:404-14. DOI:10.1016/s0076-6879(84)04107-0 |
