PIPES

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piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757

A Good buffer [1][2].

  • pKa at 25C of 6.8 (a second one at pH 3 is not of interest)
  • usable buffering range of 6.1 to 7.5
  • molecular weight 302.4
  • ΔpKa/ΔT = .0085

Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized.

Error fetching PMID 5942950:
Error fetching PMID 6717292:
  1. Error fetching PMID 5942950: [Good66]
  2. Error fetching PMID 6717292: [Blanchard84]
All Medline abstracts: PubMed HubMed
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