PLS: Difference between revisions
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== Making 2x PLS == | == Making 2x PLS == | ||
1M Tris pH 6.8 1.5ml | *1M Tris pH 6.8 1.5ml | ||
10% SDS 6.0ml | *10% SDS 6.0ml | ||
Glycerol 3.0ml | *Glycerol 3.0ml | ||
H20 3.0ml | *H20 3.0ml | ||
Bromophenol blue small dash to color | *Bromophenol blue small dash to color | ||
vortex, aliquot and freeze at -20 | vortex, aliquot and freeze at -20 | ||
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When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) | When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) | ||
Then dilute to 1x with H20 | Then dilute to 1x with H20 | ||
[[Category:Material]] and [[Category:PAGE]] | |||
Latest revision as of 14:22, 11 March 2008
Making 2x PLS
- 1M Tris pH 6.8 1.5ml
- 10% SDS 6.0ml
- Glycerol 3.0ml
- H20 3.0ml
- Bromophenol blue small dash to color
vortex, aliquot and freeze at -20
When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 and
