PLS: Difference between revisions
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When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) | When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) | ||
Then dilute to 1x with H20 | Then dilute to 1x with H20 | ||
Revision as of 08:58, 31 August 2006
Making 2x PLS
- 1M Tris pH 6.8 1.5ml
- 10% SDS 6.0ml
- Glycerol 3.0ml
- H20 3.0ml
- Bromophenol blue small dash to color
vortex, aliquot and freeze at -20
When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20
