PMSF
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==Purpose== | ==Purpose== | ||
| - | PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein | + | PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation. |
Molecular W.: 174.19 | Molecular W.: 174.19 | ||
| Line 8: | Line 8: | ||
==Use== | ==Use== | ||
| - | + | PMSF is not soluble in water, dissolve in ethanol or methanol. | |
| - | 200 mM stock solution | + | 200 mM stock solution (0.035 g/mL) is stable for months at 4°C. |
| - | A | + | A common final concentration of PMSF is 1mM of course depending on the experimental setup. |
==Safety== | ==Safety== | ||
| - | PMSF is | + | PMSF is poisonous and should be handled carefully. |
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Revision as of 18:27, 1 September 2009
Contents |
Purpose
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.
Molecular W.: 174.19
Procurement
You can buy PMSF for example at Pierce or Sigma.
Use
PMSF is not soluble in water, dissolve in ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C. A common final concentration of PMSF is 1mM of course depending on the experimental setup.
Safety
PMSF is poisonous and should be handled carefully.
