PMSF: Difference between revisions
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(New page: ==Purpose== PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations. Molecular W.: 174...) |
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==Safety== | ==Safety== | ||
PMSF is poisenes and should be handeld carefully. | PMSF is poisenes and should be handeld carefully. | ||
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Revision as of 04:00, 20 November 2007
Purpose
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations.
Molecular W.: 174.19
Procurement
You can buy PMSF for example at Pierce or Sigma.
Use
Don't try solution in water, it does not work. Take ethanol or methanol. 200 mM stock solution are stable for months at 4°C. A normal endconcentration of PMSF is probably about 1mM of course depending on the experimental setup.
Safety
PMSF is poisenes and should be handeld carefully.
