PMSF

Purpose

PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations.

Molecular W.: 174.19

Procurement

You can buy PMSF for example at Pierce or Sigma.

Use

Don't try solution in water, it does not work. Take ethanol or methanol. 200 mM stock solution are stable for months at 4°C. A normal endconcentration of PMSF is probably about 1mM of course depending on the experimental setup.

Safety

PMSF is poisenes and should be handeld carefully.